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The development of CRISPR-Cas technology is revolutionizing biology. Based on machinery bacteria use to target foreign nucleic acids, these powerful techniques allow investigators to edit nucleic acids and modulate gene expression more rapidly and accurately than ever before.Featuring contributions from leading figures in the CRISPR-Cas field, this laboratory manual presents a state-of-the-art guide to the technology. It includes step-by-step protocols for applying CRISPR-Cas-based techniques in various systems, including yeast, zebrafish, Drosophila, mice, and cultured cells (e.g., human pluripotent stem cells). The contributors cover web-based tools and approaches for designing guide RNAs that precisely target genes of interest, methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Strategies for optimizing CRISPR-Cas in each system--especially for minimizing off-target effects--are also provided.Authors also describe other applications of the CRISPR-Cas system, including its use for regulating genome activation and repression, and discuss the development of next-generation CRISPR-Cas tools. The book is thus an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and all who seek to expand their biotechnology toolkits. BioMed Central The high level of genetic diversity between individual tumors suggests that if it were to be developed a broad cancer vaccine would be unlikely to work for more than The CRISPR/Cas bacterial immune system cleaves CRISPR/Cas is a microbial immune system that is known to protect bacteria from virus infection These authors show that the Streptococcus thermophilus CRISPR/Cas Cas9 - Wikipedia Crystal structure of S pyogenes Cas9 in complex with guide RNA and its target DNA at 25 A resolution(Nishimasu et al 2014) Cold Spring Harbor Laboratory Cold Spring Harbor Laboratory - Founded in 1890 CSHL is a private non-profit institution with research programs in cancer neuroscience plant biology genomics Agilent Products Headquarters Other sites 5301 Stevens Creek Blvd Santa Clara CA 95051 United States ASM Clinical Microbiology Portal ASM's Clinical Microbiology Portal is a global forum for clinical microbiologists and immunologists Users can participate in online presentations and seek expert advice AddGene: CRISPRCas9 guide Overview of CRISPR/Cas9 The C lustered R egularly I nterspaced S hort P alindromic R epeats (CRISPR) Type II system is a bacterial immune system that has been CRISPR - Wikipedia Clustered regularly interspaced short palindromic repeats (CRISPR pronounced crisper) are segments of prokaryotic DNA containing short repetitive base sequences CRISPR-P 20: An Improved CRISPR-Cas9 - Molecular Plant Search for articles by this author Affiliations National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural University Wuhan 430070 P R China Five big mysteries about CRISPRs origins : Nature News Today much more is known about the clustered regularly interspaced short palindromic repeats that give CRISPR its name and help the CRISPRCas microbial immune
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